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Pulmonary neutrophil infiltration correlates with plasma Angiopoietin-2. Dark blue dots represent mice that received sham injury, red dots represent mice that received burn injury. ( A ) Representative H&E of lungs of sham and burn mice. ( B ) Neutrophils were counted in 6 high-powered fields in 3 mice each of sham and burn ( p < 0.0001). ( C ) Lung RT-qPCR of chemokine Cxcl1 was elevated in burn compared to sham ( p < 0.05) and ( H ) positively correlated with plasma Ang-2 ( p < 0.01). ( D – G ) Neutrophil markers ( D ) Lcn2 ( p < 0.01), ( E ) <t>Ly6g</t> ( p = 0.0737), ( F ) s100a8 ( p < 0.05), and ( G ) s100a9 ( p < 0.05) were elevated in burn compared to sham and ( I – L ) all positively correlated with plasma Ang-2 levels ( p < 0.05). For two-group analysis, significance was analyzed by unpaired t -test and correlation assessed by simple-linear regression, * p < 0.05, ** p < 0.01, **** p < 0.0001.
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Pulmonary neutrophil infiltration correlates with plasma Angiopoietin-2. Dark blue dots represent mice that received sham injury, red dots represent mice that received burn injury. ( A ) Representative H&E of lungs of sham and burn mice. ( B ) Neutrophils were counted in 6 high-powered fields in 3 mice each of sham and burn ( p < 0.0001). ( C ) Lung RT-qPCR of chemokine Cxcl1 was elevated in burn compared to sham ( p < 0.05) and ( H ) positively correlated with plasma Ang-2 ( p < 0.01). ( D – G ) Neutrophil markers ( D ) Lcn2 ( p < 0.01), ( E ) <t>Ly6g</t> ( p = 0.0737), ( F ) s100a8 ( p < 0.05), and ( G ) s100a9 ( p < 0.05) were elevated in burn compared to sham and ( I – L ) all positively correlated with plasma Ang-2 levels ( p < 0.05). For two-group analysis, significance was analyzed by unpaired t -test and correlation assessed by simple-linear regression, * p < 0.05, ** p < 0.01, **** p < 0.0001.
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Pulmonary neutrophil infiltration correlates with plasma Angiopoietin-2. Dark blue dots represent mice that received sham injury, red dots represent mice that received burn injury. ( A ) Representative H&E of lungs of sham and burn mice. ( B ) Neutrophils were counted in 6 high-powered fields in 3 mice each of sham and burn ( p < 0.0001). ( C ) Lung RT-qPCR of chemokine Cxcl1 was elevated in burn compared to sham ( p < 0.05) and ( H ) positively correlated with plasma Ang-2 ( p < 0.01). ( D – G ) Neutrophil markers ( D ) Lcn2 ( p < 0.01), ( E ) <t>Ly6g</t> ( p = 0.0737), ( F ) s100a8 ( p < 0.05), and ( G ) s100a9 ( p < 0.05) were elevated in burn compared to sham and ( I – L ) all positively correlated with plasma Ang-2 levels ( p < 0.05). For two-group analysis, significance was analyzed by unpaired t -test and correlation assessed by simple-linear regression, * p < 0.05, ** p < 0.01, **** p < 0.0001.
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Pulmonary neutrophil infiltration correlates with plasma Angiopoietin-2. Dark blue dots represent mice that received sham injury, red dots represent mice that received burn injury. ( A ) Representative H&E of lungs of sham and burn mice. ( B ) Neutrophils were counted in 6 high-powered fields in 3 mice each of sham and burn ( p < 0.0001). ( C ) Lung RT-qPCR of chemokine Cxcl1 was elevated in burn compared to sham ( p < 0.05) and ( H ) positively correlated with plasma Ang-2 ( p < 0.01). ( D – G ) Neutrophil markers ( D ) Lcn2 ( p < 0.01), ( E ) Ly6g ( p = 0.0737), ( F ) s100a8 ( p < 0.05), and ( G ) s100a9 ( p < 0.05) were elevated in burn compared to sham and ( I – L ) all positively correlated with plasma Ang-2 levels ( p < 0.05). For two-group analysis, significance was analyzed by unpaired t -test and correlation assessed by simple-linear regression, * p < 0.05, ** p < 0.01, **** p < 0.0001.

Journal: European Burn Journal

Article Title: The Role of Angiopoietin-2 in Post-Burn Pneumonia

doi: 10.3390/ebj7010001

Figure Lengend Snippet: Pulmonary neutrophil infiltration correlates with plasma Angiopoietin-2. Dark blue dots represent mice that received sham injury, red dots represent mice that received burn injury. ( A ) Representative H&E of lungs of sham and burn mice. ( B ) Neutrophils were counted in 6 high-powered fields in 3 mice each of sham and burn ( p < 0.0001). ( C ) Lung RT-qPCR of chemokine Cxcl1 was elevated in burn compared to sham ( p < 0.05) and ( H ) positively correlated with plasma Ang-2 ( p < 0.01). ( D – G ) Neutrophil markers ( D ) Lcn2 ( p < 0.01), ( E ) Ly6g ( p = 0.0737), ( F ) s100a8 ( p < 0.05), and ( G ) s100a9 ( p < 0.05) were elevated in burn compared to sham and ( I – L ) all positively correlated with plasma Ang-2 levels ( p < 0.05). For two-group analysis, significance was analyzed by unpaired t -test and correlation assessed by simple-linear regression, * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: Ly6g , Mm04934123_m1.

Techniques: Clinical Proteomics, Quantitative RT-PCR